Journal: CytoJournal
Article Title: Knocking-down annexin A3 suppresses inflammation, oxidative stress, apoptosis, and endoplasmic reticulum stress to attenuate sepsis-induced acute kidney injury in HK2 cells
doi: 10.25259/Cytojournal_64_2024
Figure Lengend Snippet: Knockdown of annexin A3 attenuates oxidative and endoplasmic reticulum stresses in lipopolysaccharide-induced human kidney 2 (HK2) cells. (a) glutathione, (b) superoxide dismutase, (c) malondialdehyde, and (d) reactive oxygen species levels in HK2 cells were quantified through enzyme-linked immunosorbent assay by using the corresponding commercial assay kits. (e and f) The protein levels of C/EBP homologous protein, glucose-regulated protein 78, inositol-requiring enzyme 1α, and activating transcription factor 6 in HK2 cells were measured through Western blot analysis. ✶ P < 0.05, ✶ ✶ P < 0.01, ✶ ✶ ✶ P < 0.001. All in vitro experiments were conducted in triplicate with at least three independent experiments. GSH: Glutathione, ANXA3: Annexin A3, NC: Negative control, siRNA: Small interfering RNA, LPS: Lipopolysaccharide, SOD: Superoxide dismutase, MDA: Malondialdehyde, ROS: Reactive oxygen species, CHOP: C/EBP homologous protein, GRP78: glucose-regulated protein 78, IRE1α: inositol requiring enzyme 1α, ATF6: activating transcription factor 6, GAPDH: Glyceraldehyde-3-phosphate dehydrogenase.
Article Snippet: The membrane was exposed to the primary antibodies anti-ANXA3 (cat. no. 11804-1-AP; Proteintech, Wuhan, China; dilution of 1:1000); anti-C/enhancer binding protein (EBP) homologous protein (CHOP) (cat. no. 15204-1-AP; Proteintech; dilution at 1:1000); anti-glucose-regulated protein 78 (GRP78) (cat. no. 11587-1-AP; Proteintech; dilution of 1:1000), anti-inositol-requiring enzyme 1α (IRE1α) (cat. no. 27528-1-AP; Proteintech; dilution of 1:1000), anti-activating transcription factor 6 (ATF6) (cat. no. 24169-1-AP; Proteintech; dilution of 1:1000), and anti-GAPDH (cat. no. Ab104788; Abclonal, Wuhan, China; dilution of 1:1000) at 4°C for 12 h after being blocked with nonfat milk (5%).
Techniques: Knockdown, Enzyme-linked Immunosorbent Assay, Western Blot, In Vitro, Negative Control, Small Interfering RNA
TargetMol is a verified supplier 
TargetMol manufactures this product 
